CBE461 Biochemistry Lab UITM Assignment Answer Malaysia

The CBE461 Biochemistry Lab at UITM (Universiti Teknologi MARA) in Malaysia offers students practical tools and fundamental techniques required for biochemistry. This course aims to provide students with hands-on experience and skills essential in the field of biochemistry. Through laboratory sessions, students will gain a deeper understanding of various biochemistry concepts and learn how to perform important experiments and analyses.

The CBE461 course emphasizes the application of theoretical knowledge to real-world scenarios, preparing students for future challenges and opportunities in biochemistry-related careers.

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Assignment Activity 1 : Perform experiments, which are related to the fundamental study covered in the biochemistry course.

Objective: 

The objective of this experiment is to study the effect of temperature on the activity of the enzyme and understand its relationship with the kinetic parameters of the reaction.

Methodology:

• Preparation of Enzyme Solution: Extract the enzyme from a suitable source and prepare a stock solution with a known concentration.
• Preparation of Substrate Solution: Prepare a substrate solution that will be used as a substrate for the enzyme-catalyzed reaction.
• Setting up Reaction Mixtures: In a series of test tubes, prepare several reaction mixtures by combining the enzyme solution and substrate solution in varying ratios.
• Temperature Treatment: Label each test tube and expose them to different temperatures (e.g., 0°C, 25°C, 37°C, 50°C, and 60°C) using a water bath or incubator.
• Incubation: Allow the reaction mixtures to incubate at their respective temperatures for a fixed period.
• Stopping the Reactions: After the incubation, stop the reactions by adding a suitable stopping reagent.
• Measurement of Reaction Rate: Use a spectrophotometer to measure the absorbance or product formation at regular intervals for each reaction mixture.
• Data Analysis: Plot the reaction rate (or product formation) against temperature to observe the relationship between temperature and enzyme activity.

Assignment Activity 2 : Develop well-structured experimental methodologies for open ended investigations

Objective: 

The objective of this investigation is to compare the antioxidant properties of various fruit extracts and understand their potential health benefits.

Methodology:

• Selection of Fruits: Choose a diverse range of fruits, such as apples, oranges, grapes, strawberries, and blueberries, among others, for the experiment.
• Fruit Extraction: Extract the antioxidants from the fruits using a suitable method like maceration or Soxhlet extraction.
• Sample Preparation: Concentrate the extracted solutions, and dilute them to obtain different concentrations of fruit extract solutions.
• Preparation of Control: Prepare a control solution without any fruit extract (e.g., using a solvent used in the extraction).
• Antioxidant Assay: Use a standard antioxidant assay, such as the DPPH (2,2-diphenyl-1-picrylhydrazyl) assay, to measure the antioxidant activity of each fruit extract and the control.
• Spectrophotometric Measurement: Use a spectrophotometer to measure the absorbance of the reaction mixtures at specific time points.
• Data Collection: Record the absorbance values and calculate the percentage of antioxidant activity for each fruit extract.
• Statistical Analysis: Analyze the data using appropriate statistical methods to determine any significant differences between the fruit extracts and the control.

Assignment Activity 3 : Evaluate the experimental results with the theories and interpret the data.

Objective: 

The objective of this experiment is to determine the effect of pH on the activity of the enzyme and identify its optimal pH for maximum activity.

Methodology:

• Enzyme and Substrate Preparation: Obtain the enzyme and prepare a substrate solution for the reaction.
• pH Adjustment: Set up a series of reaction mixtures with the enzyme and substrate, each at a different pH value (e.g., pH 4, 6, 7, 8, and 10).
• Incubation: Incubate the reaction mixtures at a constant temperature for a fixed duration.
• Enzyme Activity Measurement: Use a suitable method to measure the enzyme activity, such as monitoring the product formation or substrate depletion using a spectrophotometer.
• Data Collection: Record the enzyme activity values for each pH condition.
• Data Analysis: Plot the enzyme activity against pH to identify the trend in enzyme activity concerning pH.
• Interpretation: Analyze the results to determine the optimal pH for the enzyme’s activity and discuss the factors influencing the enzyme’s performance at different pH levels.

Remember to provide detailed descriptions of the procedures, equipment, and measurements used in the experiments. Additionally, ensure proper safety precautions are taken throughout the experiments.

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