BIO552 Analytical Biochemistry UITM Assignment Answer Malaysia

BIO552 Analytical Biochemistry” at UITM Malaysia is a comprehensive course that focuses on preparing students for the analysis of proteins and biomolecules using various techniques. These analytical methods include chromatography, electrophoresis, spectroscopy, and mass spectrometry. The course also covers the practical application of these techniques in current bio science research.

Assessment in this course comprises traditional final examinations, tests, quizzes, assignments, and lab reports. Successful completion of BIO552 Analytical Biochemistry is crucial for students aspiring to pursue careers as laboratory technologists or research scientists in fields like biochemistry, biotechnology, and molecular biology.

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Assignment Brief 1 : Display various bioanalytical techniques in analyzing samples [P4]

In this assignment, you are required to showcase different bioanalytical techniques used in the analysis of biological samples. The focus should be on providing a comprehensive overview of various methods employed to study and quantify biological molecules. Some of the key techniques you can include are:

• Spectroscopy: Explain different spectroscopic techniques like UV-Vis spectrophotometry, fluorescence spectroscopy, and infrared spectroscopy, highlighting their applications in analyzing biomolecules.
• Chromatography: Discuss various chromatographic techniques such as high-performance liquid chromatography (HPLC), gas chromatography (GC), and thin-layer chromatography (TLC), emphasizing their utility in separating and quantifying biomolecules.
• Mass Spectrometry: Describe the principles of mass spectrometry and its applications in identifying and quantifying biomolecules, including proteins, peptides, and metabolites.
• Electrophoresis: Explain gel electrophoresis, capillary electrophoresis, and their significance in separating and analyzing proteins, nucleic acids, and other biomolecules based on their size and charge.
• Immunoassays: Detail various immunoassay techniques like enzyme-linked immunosorbent assay (ELISA), radioimmunoassay (RIA), and their role in detecting and quantifying specific antigens or antibodies in biological samples.
• NMR Spectroscopy: Introduce nuclear magnetic resonance (NMR) spectroscopy and its applications in structural analysis of biomolecules.
• Microscopy: Discuss different microscopy techniques, such as fluorescence microscopy, confocal microscopy, and electron microscopy, and their uses in visualizing cellular and subcellular structures.

Remember to provide examples of real-life applications of these techniques and how they contribute to advancements in analytical biochemistry and biomedical research.

Assignment Brief 2 : Solve scientific problems in analytical biochemistry [C3].

In this assignment, you will be presented with scientific problems related to analytical biochemistry, and your task is to demonstrate your problem-solving skills in resolving these issues. The problems may involve the analysis of biological samples, data interpretation, troubleshooting experimental procedures, or devising strategies for specific bioanalytical challenges.

Some potential problem scenarios could include:

• Designing an experimental protocol for quantifying a specific protein or metabolite in a complex biological sample using appropriate bioanalytical techniques.
• Analyzing and interpreting data obtained from an HPLC or GC experiment, identifying peaks, and calculating the concentrations of the analytes.
• Troubleshooting issues in an immunoassay experiment, such as minimizing background noise or enhancing sensitivity.
• Analyzing NMR spectra of biomolecules to determine their structures.
• Evaluating the performance of different bioanalytical techniques for a given sample type and selecting the most suitable method based on their advantages and limitations.

Ensure that you apply critical thinking and scientific reasoning to solve these problems effectively. Provide step-by-step explanations of your solutions and rationale behind your choices. Additionally, if there are multiple viable solutions, compare and contrast them to showcase your understanding of the subject matter.

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